Searchable abstracts of presentations at key conferences on calcified tissues

ba0005p149 | Cell biology: osteoblasts and bone formation | ECTS2016

Enhancement of bone ultra structure preservation using high-pressure freezing and microwave-assisted fixation

Daghma Diaa Eldin S. , Heiss Christian , Khassawna Thaqif El

Despite the improvement in bone’s molecular, metabolic and live-cell imaging, histological investigation remains most crucial in bone biology for diagnostic and research purposes. Therefore, fixation of bone samples – especially for electron microscopy investigation – is critical to the ultrastructural analysis. Up to date, chemical fixation of bone tissue is performed at room temperature resulting in a compromised ultrastructure of bone sample. In this study, w...